glutathione S transferase
- glutathione S transferase
-
Enzyme that will couple
glutathione to
a xenobiotic as the first step in removal. Now very commonly used as
a fusion with
a gene of interest that is being expressed in
a bacterial system. The fusion construct can be purified easily from lysate by
passage down
a glutathione affinity column, the purified construct then being eluted with glutathione. The
GST can then be cleaved proteolytically from the
protein of interest though often the complete
fusion protein can be used.
Dictionary of molecular biology.
2004.
Look at other dictionaries:
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Glutathione S-transferase — The glutathione S transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. This is a… … Wikipedia
Glutathione S-transferase, C-terminal domain — Pfam box Symbol = GST C Name = Glutathione S transferase, C terminal domain width = caption = Pfam= PF00043 InterPro= IPR004046 SMART= Prosite = SCOP = 2gst TCDB = OPM family= 139 OPM protein= 1z9h PDB=PDB3|1gnwA:111 206 PDB3|1bx9A:111 206… … Wikipedia
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Microsomal glutathione S-transferase 1 — Identifiers Symbols MGST1; GST12; MGC14525; MGST; MGST I External IDs … Wikipedia
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glutathione — A tripeptide of glycine, l cysteine, and l glutamate, with l glutamate having an isopeptide bond with the amino moiety of l cysteine. G. has a wide variety of roles in a cell; it is the most prevalent non protein thiol. G. disulfide (GSSG)… … Medical dictionary
Glutathion-S-Transferase — Enzymklassifikation EC, Kategorie … Deutsch Wikipedia
